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reduces IgE binding ability of allergenic egg white proteins
Sen LI, Marina OFFENGENDEN, Michael G. GÄNZLE, Jianping WU
《农业科学与工程前沿(英文)》 2018年 第5卷 第3期 页码 373-381 doi: 10.15302/J-FASE-2018210
Egg white proteins are one of the major allergens. The objective of this study was to investigate the effect of Aspergillus oryzae cultivation on IgE binding ability of egg white proteins. Effect of A. oryzae on egg white proteins was determined using ninhydrin method, SDS-PAGE, ELISA, fluorescence FITC labeling, MALDI-TOF-MS and LC-MS/MS analysis. Adding mycelium of A. oryzae ATCC 1011 and 16868 substantially reduced the IgE binding ability of acidified egg white after 24 h incubation. The binding capacity of egg white proteins to IgE in plasma from four egg allergy patients was almost completely lost after incubation with mycelium of ATCC 16868. Results from SDS-PAGE, free amino acid analysis, MALDI-TOF-MS and LC-MS/MS indicated that there was no substantial protein degradation during incubation. Therefore, the reduction of IgE binding ability of egg white proteins during A. oryzae treatment was probably due to a loss of ~1700 Da mass including a fragment of the ovomucoid N terminus.
关键词: Aspergillus oryzae egg allergy egg white proteins IgE-binding ability ovomucoid
Xiaojuan NIU, Shuyi QIU, Yuangen WU, Jie YUAN, Yingying XU
《化学科学与工程前沿(英文)》 2011年 第5卷 第1期 页码 122-125 doi: 10.1007/s11705-010-0533-6
关键词:
soybean
Enhanced enzymatic removal of anthracene by the mangrove soil-derived fungus,
Paul Olusegun Bankole, Kirk Taylor Semple, Byong-Hun Jeon, Sanjay Prabhu Govindwar
《环境科学与工程前沿(英文)》 2020年 第14卷 第6期 doi: 10.1007/s11783-020-1292-3
关键词: Aspergillus sydowii Anthracene Biodegradation Polycyclic aromatic hydrocarbons
利用基因组特异性引物和Cas12a介导技术快速检测稻瘟菌小麦致病型 Article
康厚祥, 彭烨, Kangyu Hua, 邓雨飞, Maria Bellizzi, Dipali Rani Gupta, Nur Uddin Mahmud, Alfredo S. Urashima, Sanjoy Kumar Paul, Gary Peterson, 周益林, 周雪平, Md Tofazzal Islam, 王国梁
《工程(英文)》 2021年 第7卷 第9期 页码 1326-1335 doi: 10.1016/j.eng.2020.07.016
麦瘟病由稻瘟菌小麦致病型(MoT)引起,是一种存在于南美洲和孟加拉国的毁灭性病害。由于MoT通常不会在抽穗期之前使小麦显现染病症状,但在抽穗期其侵染会一直加剧,因此仅根据肉眼观察来使用杀菌剂是无效的。为了开发一种在苗期和营养期准确、灵敏检测MoT以控制病害蔓延的方法,我们对来自巴西的两个MoT分离株的基因组进行了测序,并确定了两个DNA片段:MoT-6098 和MoT-6099,它们存在于麦瘟病菌(MoT)基因组中,但不存在于感染稻瘟病菌(MoO)的水稻基因组中。利用聚合酶链反应(PCR),我们在来自南美洲和孟加拉国的53 株MoT和MoO分离株中证实了这两对标记引物的特异性。为了测试这两对标记引物的有效性,我们首先建立了一种环介导等温扩增(LAMP)方法。在等温条件下,这种方法在不使用常规PCR仪器的情况下也能检测MoT。随后,我们使用Cas12a 蛋白及导向RNA(gRNA)靶向MoT-6098 和MoT-6099 序列,两个靶标被识别后,均能激活Cas12a 非特异切割单链脱氧核糖核酸酶(ssDNAase)的活性,我们将依赖靶向Cas12a 激活ssDNase 活性,同时将重组酶聚合酶扩增(RPA)和核酸侧流免疫分析法(NALFIA)相结合,开发出一种准确、灵敏且经济高效地检测受感染小麦植株中MoT特异性DNA序列的方法。这种新技术可应用于田间麦瘟病和其他重要植物病害的快速检测。
标题 作者 时间 类型 操作
reduces IgE binding ability of allergenic egg white proteins
Sen LI, Marina OFFENGENDEN, Michael G. GÄNZLE, Jianping WU
期刊论文
Highly effective extraction of oil from soybean by pretreatment of solid-state fermentation with fungi
Xiaojuan NIU, Shuyi QIU, Yuangen WU, Jie YUAN, Yingying XU
期刊论文
Enhanced enzymatic removal of anthracene by the mangrove soil-derived fungus,
Paul Olusegun Bankole, Kirk Taylor Semple, Byong-Hun Jeon, Sanjay Prabhu Govindwar
期刊论文